Summary and Significance

One of the most important features of SynAppBIo’s technology is our ability to control both the synthesis enzymes and their enzyme cofactors to permit their continuous function over thousands of cycles (see the NAE Bridge article on industrial and natural cycles). This article by the SynAppBio founders describes much of the scientific and engineering basis of SynAppBio’s strategy to achieve this control and what it’s significance should be to the biotechnology industry.

Abstract Summary

Direct electrochemical reduction of enzyme cofactors like NAD(P)
invariably produces unwanted cofactor side products, resulting in unproductive loss of input NAD(P). We review various literature strategies for mitigating unwanted product formation by electrochemical cofactor regeneration systems and offer insight as to how a successful electrochemical bioreactor system could be constructed to engineer efficient 1,4-NAD(P)H 2-dependent enzyme reactions of interest to the industrial biocatalysis community. The embedded link is to the publisher, Elsevier. If you are not able to access the publication, please fill out a contact form and we may be able to provide you with a single copy for your use.